Status : Published (Under review)

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Abstract

This document specifies a method for the determination of fish acute toxicity using the permanent cell line from rainbow trout (Oncorhynchus mykiss) gill, RTgill-W1. Cells in confluent monolayers in 24-well tissue culture plates are exposed to water samples, such as surface waters or different kinds of effluents, or to chemicals for 24 h and, thereafter, cell viability is assessed based on fluorescent cell viability indicator dyes (see 4.1). Data are then expressed as a percentage of unexposed control and toxicity quantified based on the percentage of cell viability versus the percentage of effluent or the chemical concentration in response curves (see Clause 9).

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General information

  • Status
     : Published
    Publication date
     : 2019-04
    Stage
    : International Standard under systematic review [90.20]
  • Edition
     : 1
    Number of pages
     : 38
  • Technical Committee :
    ISO/TC 147/SC 5
    ICS :
    13.060.70 
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Life cycle

Sustainable Development Goals

This standard contributes to the following Sustainable Development Goals

12
Responsible Consumption and Production
3
Good Health and Well-being
15
Life on Land
6
Clean Water and Sanitation

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