ISO/TS 21569-3:2020

This standard was last reviewed and confirmed in 2023. Therefore this version remains current.

Abstract

This document describes a procedure for the detection of the DNA transition sequence between the 35S promotor (P35S) from Cauliflower mosaic virus and a modified phoshinothricin-acetyltransferase gene (pat) from Streptomyces viridochromogenes. The P35S-pat construct is frequently found in genetically modified plants with tolerance for phosphinothricin-containing herbicides. The P35S-pat construct specific method is based on a real-time PCR and can be used for qualitative and quantitative screening purposes. For identification and quantification of a specific event, a follow-up analysis can be carried out.

This document is applicable to the analysis of DNA extracted from foodstuffs. It can also be suitable for the analysis of DNA extracted from other products such as feedstuffs and seeds. The application of this method requires the extraction of an adequate quantity and quality of amplifiable DNA from the relevant matrix.

General information

Status
: Published

Publication date
: 2020-06
Edition
: 2

Number of pages
: 14
Technical Committee
: ISO/TC 34/SC 16 Horizontal methods for molecular biomarker analysis
ICS
:
67.050 General methods of tests and analysis for food products