Draft International Standard
ISO/DIS 13136-1
Microbiology of the food chain — Detection, isolation and characterization of Shiga toxin-producing Escherichia coli (STEC) — Part 1: Horizontal method for the detection and isolation of Shiga toxin-producing Escherichia coli (STEC)
Reference number
ISO/DIS 13136-1
Edition 1
International Standard
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ISO/DIS 13136-1
This Draft International Standard is in the enquiry phase with ISO members.
Will replace ISO/TS 13136:2012

ISO/DIS 13136-1

ISO/DIS 13136-1
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This standard describes the detection and isolation of Shiga toxin-producing Escherichia coli (STEC). The procedure includes the detection by Real Time PCR of the major virulence genes of STEC, stx1 and stx2 (Reference [2]) in enrichment cultures. Isolation of STEC from the enrichment culture is attempted if one or both stx genes are detected in the screening. This document is applicable to — products intended for human consumption, — products intended for animal feeding, — environmental samples in the area of food and feed production, handling, and — samples from the primary production stage.

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This standard contributes to the following Sustainable Development Goal

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